By Yangyang Yang
In this e-book, the writer bargains in most cases with issues: (1) single-molecule visualization of switching behaviors within the DNA nanoframe method using other kinds of molecular switches by using high-speed atomic strength microscope (AFM); (2) building of photocontrollable DNA nanostructures in programmed styles and direct visualization of the dynamic assembling approach. right here, high-speed AFM was once hired to watch the dynamic pursuits of unmarried molecules. in comparison to a standard single-molecule research technique, reminiscent of fluorescence spectroscopy or electron microscopy, high-speed AFM makes attainable the real-time statement of molecule behaviors. DNA nanostructures have been designed and assembled as scaffolds to include biomolecules. The observations have been conducted below strong stipulations with out advanced pretreatment. furthermore, the photoresponsive molecules have been effectively assembled into round a hundred nm-sized DNA nanostructures. The assembly/disassembly of nanostructures should be regulated reversibly by way of photoirradiation. This ebook explains how DNA origami has progressively turn into a useful gizmo for the research of biochemical interactions in outlined nanospace. It additionally exhibits the potential for DNA nanostructures appearing as nanodevices for software in organic platforms, serving as a very good creation to uncomplicated DNA nanotechnology.
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Extra info for Artificially Controllable Nanodevices Constructed by DNA Origami Technology: Photofunctionalization and Single-Molecule Analysis
Chem. Int. Ed, 51/42. Copyright (c)  [copyright owner as speciﬁed in the Journal]. (http://onlinelibrary. 5 Direct Observation of Association of Photoresponsive Domains in Nanoframe Under Visible Light Irradiation Under UV irradiation, the dissociation behaviors of single pair of photoresponsive domain on DNA nanoframe were directly visualized in real-time. Next it is tried to observe the reverse hybridization movements with the irradiation of visible light. The sample pretreated with UV light was loaded on the mica surface and the expected nanoframes in separated-shape were imaged.
Mohri K, Nishikawa M, Takahashi N, Shiomi T, Matsuoka N, Ogawa K, Endo M, Hidaka K, Sugiyama H, Takahashi Y, Takakura Y (2012) ACS Nano 6:5931–5940 66. Schüller VJ, Heidegger S, Sandholzer N, Nickels PC, Suhartha NA, Endres S, Bourquin C, Liedl T (2011) ACS Nano 5:9696–9702 67. 1 Introduction Hybridization is the process that two complementary single-stranded nucleic acid molecules (DNA or RNA) associate together into a single double-stranded helix through speciﬁc base pairing. This process is reversible and the duplex can be controlled to dissociate by regulating the reaction conditions.
This technique provides a viable tool to decipher the DNA strand transformation processes during state transition. Previously, our lab have developed a series of frame-shaped DNA origami tethered with single DNA strands functionalized with DNA motifs, such as G-telomeric repeats,  photoresponsive oligonucleotides,  and B-Z DNA transiting strands . Such DNA nanostructures have been proved to be able to observe nanomechanical movements in response to one speciﬁc trigger using high-speed AFM at single molecule level and in real-time fashion .