Chromatographic Fingerprint Analysis of Herbal Medicines by Hildebert Wagner, Rudolf Bauer, Dieter Melchart, Pei-Gen

By Hildebert Wagner, Rudolf Bauer, Dieter Melchart, Pei-Gen Xiao, Anton Staudinger

Volume III of this guide offers an summary of the analytical research of 23 extra chinese language natural medicines, that are most typically utilized in conventional chinese language medication. including Volumes I and II this present quantity represents the main accomplished review to analytical experiences of these natural medicinal drugs. the standard evidence of the research meets the traditional of the ecu Drug Regulatory Authority. The authors seek advice from the bioactive components, pharmacological and organic actions of all unmarried natural medicinal drugs, in addition to their healing functions. Analytical equipment utilized are defined in detail.

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Additional info for Chromatographic Fingerprint Analysis of Herbal Medicines Volume III: Thin-layer and High Performance Liquid Chromatography of Chinese Drugs

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5 g 2,4-dinitrophenylhydrazin are dissolved in 20 ml sulphuric acid (25 %), filled up with water to 100 ml and filtered. After spraying with 10 ml, the plate is evaluated after 10 min in VIS. 3. Anisaldehyde – Sulphuric acid reagent (Fig. 5 ml anisaldehyde is mixed with 10 ml glacial acetic acid, followed by 85 ml methanol and 5 ml concentrated sulphuric acid, in that order. The plate is sprayed with 10 ml, heated at 100 °C for 5 min, then evaluated in VIS and under 366 nm. Note: The reagent has only limited stability and is no longer useable when the colour has turned to red-violet.

0 0 5 10 15 20 30 25 35 40 45 Retention time (min) Fig. 0 0 5 10 15 20 25 30 35 40 45 Retention time (min) Fig. 0 200 220 240 260 280 300 320 340 360 380 400 Wavelength (nm) Fig. 0 % of volatile oil, according to the Chinese Pharmacopoeia [1]. Conclusion The identity of Rhizoma Cyperi can be easily determined by TLC- and HPLC-analysis using MeOH-extract or essential oil by means of the characteristic α-β-cyperone dublett in HPLC. References 1. Pharmacopoeia of the People’s Republic of China, English Edition, vol.

20 μm and injected into the HPLC apparatus. 1 % H3PO4/1 l. dist. g. g. Procyanidin B2) Hyperosid Isoquercitrin Description of Fig. 0 2–3 main peaks inclusive several minor peaks which can be assigned to several procyanidins (catechins). They are characterized through UV-spectra with endabsorption and a small inflexion at 276 nm. Peak 6 might be the flavonol-galactoside hyperoside. • The extract of Crataegi folium (sample 13) is characterized by chlorogenic acid (1), the bulk of procyanidins (2), the main flavonol-/and flavon O- and C-glycoside vitexin (3), rutin (4), hyperosid (6) and traces of isoquercitrin (7), and procyanidin B2 (5).

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